Performance of blast searches in a badly sampled group using the COI-5P sequence of Cryptomonas curvata strain CCAC 0080 as a query (570 nt): rankings and statistics of the discontiguous megablast search.

Except for C. ovata and G. theta, no other cryptophytes among first 100 hits; all others: stramenopile sequences, predominantly brown algae. Discontiguous megablast and blastn resulted in the same ranking among first hits. No changes were observed with blastn word size reduced to 7. The R. salina cox1 sequence was interrupted by a group 2 intron in the COI-5P region, thus yielded two alignments. Its low ranking did not allow for viewing of pairwise alignments. Query coverage and expectancy values according to list view, but score bits, identities, and gaps statistics have been taken from a search constrained to cryptomonads (expectancy values change under these circumstances).