five

CelFi: A Rapid and Robust Validation Strategy for Pooled CRISPR Screens

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NIAID Data Ecosystem2026-05-02 收录
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https://www.ncbi.nlm.nih.gov/sra/SRP486502
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A CRISPR-based method for hit validation that tests the effect of a genetic perturbation on cell fitness. By editing target loci and monitoring out-of-frame (loss-of-function) allelic profiles over time, we have created a Cellular Fitness (CelFi) assay that can elucidate cellular vulnerabilities and verify hits from pooled CRISPR screens.
创建时间:
2025-01-17
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