The Regulatory Network and Role of the circRNA-miRNA-mRNA ceRNA Network in the Progression and the Immune Response of Wilms Tumor based on RNA-seq

Circular RNA (circRNA), which is a newly discovered non-coding RNA, has been documented to play important roles in miRNA sponges, and the dysregulation of which is involved in cancer development. However, circRNA expression profiles and their role in initiation and progression of Wilms tumor (WT) remain largely unclear at present. Here, we used paired WT samples and high-throughput RNA sequencing to identify differentially expressed circRNAs. Overall design: we used 4 paired WT samples and high-throughput RNA sequencing to identify differentially expressed circRNAs. A complete circRNA-miRNA-mRNA network was then constructed by miRanda prediction tool. Besides these, we selected a key circRNA, circEYA1, for function validation.