Functional unloading leads to metabolic and functional disorders in FAPs in vitro

In our study, we used hindlimb unloading rat model to study fibro-adipogenic progenitor cells (FAPs) condition in muscle atrophy. We have purified FAPs from m. soleus from control rats and after 7 and 14 days of immobilization (HS7, HS14). Also, we performed adipogenic differentiation of FAPS in vitro during 3 days.本研究采用后肢去负荷大鼠模型，探究肌肉萎缩过程中成纤维脂肪祖细胞（fibro-adipogenic progenitor cells, FAPs）的状态变化。我们分别从对照组大鼠以及经后肢去负荷7天（HS7）、14天（HS14）处理的模型大鼠的比目鱼肌（m. soleus）中分离纯化FAPs。此外，我们还在体外对FAPs进行了为期3天的成脂分化诱导。