Waterlogging transcriptome analysis revealed two mechanisms of canna's response to waterlogging stress

Waterlogging is a phenomenon caused by excessive soil moisture, which often occurs during the cultivation of canna plants, leading to a lack of oxygen in the root zone. However, there are few studies on the potential mechanism of canna in response to waterlogging stress. In this study, the transcriptomes were compared by Illumina RNA sequencing technology, and the differential morphological, physiological and gene expression responses of the roots of waterlogging tolerant cultivar 'Murray' and waterlogging cultivar 'QBJY' after 0, 1, 3 and 5 days of waterlogging stress were studied, and the possible mechanism of waterlogging of Cabbage beauty was elucidated. The results showed that 'MH' had a stronger response at the transcriptional level than 'QBJY', and the differentially expressed genes of the two cultivars were mainly enriched in hormone signal transduction, MAPK signaling pathway, starch and sucrose metabolism, glycolysis/gluconeogenesis, and antioxidant enzymes in KEGG analysis. Among the 10 transcription factor families screened, ERFs had the largest number of transcription factors, and their family members ERF071-like, RAP2-4, RAP2-9 and DREB3 were significantly up-regulated by flooding in 'Ink Red', and the expression degree was significantly higher than that of 'Qianbei Banana Taro'. In addition, 51 differential genes with significant up-regulated expression at least one flood treatment time point were screened out as candidate genes for waterlogging tolerance, and the up-regulated expression intensity of these genes in 'Ink Red' was higher than that of 'Qianbei Banana Taro', and they played an important role in the waterlogging tolerance of Canna with different expression patterns. Our findings provide insights into the factors affecting waterlogging tolerance of Cabbage and provide a basis for further research on molecular breeding of different Cabbage varieties and important plant traits.