Experimental dataset on Microstructured cellulose acetate biointerfaces regulate mesenchymal stem cell response and promote osteogenic commitment

This dataset was generated as part of NFFA-Europe/FORTH activities to support the physicochemical and surface-mechanical characterization of cellulose acetate (CA) biointerfaces developed for mesenchymal stem cell response and osteogenic commitment studies. Atomic force microscopy (AFM) measurements were performed on CA replicas with three different surface topographies: Flat, Low Roughness (LR), and High Roughness (HR). The measurements were used to evaluate surface morphology, topographical features, local stiffness, adhesion, and reduced Young’s modulus of the CA biointerfaces. The dataset includes AFM topographic images, phase/topography-related outputs, nanomechanical maps, force-distance curve data, and derived image files corresponding to the Flat, Low Roughness, and High Roughness CA samples. These data support the AFM-based analysis presented in the article “Microstructured cellulose acetate biointerfaces regulate mesenchymal stem cell response and promote osteogenic commitment”, specifically the AFM characterization and Figure 2.