Single-cell transcriptome analysis of Myt1l mutant mouse cortices at birth.

We examined the effect of Myt1l deficiency in the cortices of mice at birth on the single cell level. Homozygous Myt1l deficiency resulted in postnatal lethality, and mutant mice presented increased expression of non-neuronal genes in neurons. Single-cell RNA sequencing of Myt1l (+/+; WT), Myt1l (+/-; HET), and Myt1l (-/-; HOM) mouse cortices at birth.本研究在单细胞层面探究了新生小鼠大脑皮层中Myt1l基因缺失的生物学效应。纯合型Myt1l基因缺失会导致小鼠出生后致死，且突变小鼠的神经元中呈现非神经元基因表达上调的现象。本次研究对出生时的Myt1l野生型（+/+; WT）、杂合型（+/-; HET）及纯合型（-/-; HOM）小鼠的大脑皮层开展了单细胞RNA测序。