Figure.3.PBMC.Donor.1
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http://flowrepository.org/id/FR-FCM-ZZU4
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资源简介：
To interrogate nuclear import of NFkB across cell subsets in human PBMCs.
Conclusion:
NFkB translocated to the nucleus with different kinetics across cell subsets.
Notes:
This experiment is the first of two donors used in the analysis shown in Figure 3 of the SLA manuscript. There were three timepoints: 5, 15, and 30 minutes across two different NFkB-stimulatory ligands: TNFa and LPS. Each of these timepoints had an unstimulated control. All antibodies for this experiment were extensively titrated prior to use, along with SLA reagents. PBMCs were stimulated and processed fresh (as opposed to viably frozen). Negative controls included both unstimulated cells, and an SLA workup not using primary antibodies (no signal expected). The positive control was the interaction between double-strand DNA and histone H3 antibodies.
创建时间：
2017-01-01
