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Quantification of the Amount of CID Depletion in cid Mutant Embryos

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Figshare2015-12-02 更新2026-05-11 收录
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https://figshare.com/articles/dataset/_Drosophila_CENP_A_Mutations_Cause_a_BubR1_Dependent_Early_Mitotic_Delay_without_Normal_Localization_of_Kinetochore_Components/152900
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Stage 15 cid/cid and cid/CyO embryos were stained for CID and PROD. PROD was present in a punctate pattern in both genotypes. Estimation of the amount of CID depletion was preformed by comparing the ratio of total CID staining to PROD staining in five different mutant and heterozygous embryos. From this analysis we estimate that 90%�C100% of CID protein is depleted in mutant embryos. (4.0 MB PDF)
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2015-12-02
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