MIS-C RNA immune gene expression data

Quantitative PCR results for 80 immune genes of interest from patients with multisystem inflammatory syndrome in children (MIS-C) and other controls: healthy non-inflammatory paediatric controls, Kawasaki disease, lupus, and other inflammatory conditions. Data is presented as 1/deltaCT values computed for each transcript relative to mean of three reference transcripts (ACTR3, CDC42, USF2). Transcripts of interest were pre-amplified using a pool of 96 TaqMan primer-probe assays. Pre-amplified cDNA, along with an internal positive control and a no-template (water) control, and the 96 primer-probe assays, were loaded into a microfluidic 96.96 Gene Expression Integrated Fluidic Circuit (Fluidigm) and gene expression of individual transcripts was then quantified by quantitative real time PCR performed on a BioMark HD (Fluidigm) instrument. The following parameters were applied for extracting cycle threshold (CT) values: Linear (Derivative) baseline correction, Quality Threshold of 0.3 and Auto (Global) for Ct Threshold Method using Fluidigm software version 4.5.2. 

Column headings and descriptions
A1 - Sample_code - deidentified sample identifier
B1 - Disease_group - disease group (healthy control, juvenile systemic lupus erythematosus, Kawasaki disease, inflammatory (sepsis) control, or MIS-C
C1 - Treatment - treatment by the timepoint (none, or various combinations of IVIG and/or medrol)
D1 - Treat_time_(hrs_since_1st_treatment) - time in hours since the first treatment (described in C1)
E1-CF1 - [gene names] - expression of the gene name indicated in the column heading

针对儿童多系统炎症综合征（MIS-C）及其他对照组（健康非炎症儿科对照组、川崎病、红斑狼疮及其他炎症性疾病）的80个免疫相关基因的定量PCR结果：数据以1/deltaCT值呈现，该值是针对每个转录本相对于三个参考转录本（ACTR3、CDC42、USF2）平均值计算得出。感兴趣的转录本通过96个TaqMan引物-探针检测混合池进行预扩增。预扩增的cDNA，连同内部阳性对照和空白模板（水）对照，以及96个引物-探针检测，被加载到微流控96.96基因表达集成流体电路（Fluidigm）中，并通过在BioMark HD（Fluidigm）仪器上进行的定量实时PCR对单个转录本的基因表达进行量化。提取循环阈值（CT）值时应用了以下参数：线性（导数）基线校正、质量阈值0.3以及使用Fluidigm软件版本4.5.2的自动（全局）CT阈值方法。列标题及描述：A1 - 样本代码 - 匿名样本标识符；B1 - 疾病组 - 疾病分组（健康对照组、幼年性系统性红斑狼疮、川崎病、炎症性（脓毒症）对照组或MIS-C；C1 - 治疗 - 至指定时间点的治疗方案（无或IVIG和/或美洛昔康的多种组合）；D1 - 治疗时间（自首次治疗起的小时数）- 自首次治疗起的时间，如C1所述；E1-CF1 - [基因名称] - 列标题中指示的基因表达。