METTL3 mediated m6A mRNA methylation regulates neutrophil activation through targeting TLR4 signaling (RNA-Seq)

To investigate the effect of METTL3 function in the regulation of neutrophil activation, we established neutrophil METTL3 knock out mice by crossing METTL3flox/flox mice with Lyzm-cre mice. We then performed gene expression profiling analysis using data obtained from RNA-seq of isolated neutrophil from METTL3f/f and METTL3f/f Lyzm-Cre mice with or without intraperitoneal injection of lipopolysaccharide (LPS) treatment. Comparative gene expression profiling analysis of RNA-seq data for METTL3flox/flox neutrophil and METTL3flox/flox-Lyzmcre neutrophil.为探究甲基转移酶样3（methyltransferase-like 3, METTL3）在中性粒细胞（neutrophil）活化调控中的功能效应，我们通过将METTL3 flox/flox小鼠与Lyzm-Cre小鼠杂交，构建了中性粒细胞特异性METTL3敲除小鼠。随后，我们针对分离自METTL3f/f与METTL3f/f Lyzm-Cre小鼠的中性粒细胞，结合脂多糖（lipopolysaccharide, LPS）腹腔注射处理与未处理的分组，基于RNA测序（RNA-seq）获取的转录组数据开展基因表达谱分析。本数据集针对METTL3flox/flox中性粒细胞与METTL3flox/flox-Lyzmcre中性粒细胞的RNA测序数据，开展对比基因表达谱分析。