Single crystal x-ray diffraction data set for human GABARAP revealing an extended N-terminal conformation

Human GABARAP (gamma-aminobutyric acid receptor-associated protein) belongs to the human autophagy-related protein 8 (hATG8) family which is implicated in various aspects of macroautophagy and other vesicle trafficking pathways. The family consists of six homologues classified into two subfamilies, the GABARAP subfamily and the microtubule-associated protein 1 light chain 3 (MAP1LC3) subfamily containing three proteins each. The human GABARAP protein was crystallised in the presence of a small-molecule compound solubilised with DMSO. The crystal selected for data collection was obtained using PEG 4000 and sodium acetate as precipitating agents in a TRIS buffer. Glycerol was added as cryo-protectant during crystal preparation. Single crystal x-ray diffraction data extending to a diffraction limit of 1.68 Å were obtained on beamline ID23-1 at the European Synchrotron Radiation Facility (ESRF; Grenoble, France), using an x-ray wavelength of 0.8997 Å. The crystal structure reveals an extended N-terminal conformation.