RMSD and Trp assays for "Structural transitions in kinesin minus-end directed microtubule motility"

This entry is for our manuscript, "Structural transitions in kinesin minus-end directed microtubule motility" by Satoki Shibata*, Matthew Y. Wang*, Tsuyoshi Imasaki*, Hideki Shigematsu, Diego Ugarte La Torre, Yuanyuan Wei, Chacko Jobichen, Hajime Hagio, J. Sivaraman, Yuji Sugita, Sharyn A. Endow & Ryo Nitta. *Equal contribution Corresponding Authors: Tsuyoshi Imasaki*, Sharyn A. Endow, Ryo Nitta The deposited datasets are 1) beta-strand residue all-atom RMSD for kinesin-14 NcdY485K ADP and ADP + free Pi nucleotide states and 2) fluorimeter assays of NcdY485K intrinsic Trp fluorescence changes upon addition of free Pi . The data are presented in Fig. 8e, and Fig. 9b,c & Supplementary Fig 8, respectively. Files are named for the figures in which the data are shown. A computer script for the RMSD analysis and methods for the fluorimeter assays and data analysis are included.