MOESM1 of Asymmetric DNA methylation of CpG dyads is a feature of secondary DMRs associated with the Dlk1/Gtl2 imprinting cluster in mouse

Additional file 1. Data used for statistical analyses of DNA methylation levels at the Gtl2- and IG-DMRs. The numerical data used to perform Mann-Whitney U tests and the resulting P values are contained in this file. Data from the Gtl2-DMR 5’ region, Gtl2-DMR 3’ region and the IG-DMR are presented in separate sheets. Within each sheet, data from each of the developmental stages are presented in chronological order, as they are in the Results, Figures, and Tables. Each sheet presents the information for a specific locus, tissue, cross (maternal allele x paternal allele), and parental allele analyzed, as indicated in columns A-D. % methylation (column F) was calculated by dividing the number of methylated CpG sites observed in a given subclone (column E) by the total number of CpG sites analyzed within the subclone; the raw data used to make these calculations are found in Figs. 2, 3 and 4. For the Gtl2-DMR 3’ region and the IG-DMR, P values were calculated independently for BxC samples vs. CxB samples. In addition, P values were calculated for the combined BxC + CxB samples, as some of the BxC and CxB sample sizes were too small to accurately perform Mann-Whitney U tests. Data presented in sheets labelled “grouped” combine subclones derived from the same PCR with identical DNA methylation patterns and identical sequences as a single sample, while every subclone analyzed is treated as an independent sample in sheets labelled “ungrouped”.