Properties of circular-permutated mCherry variants.

aAll circular-permutated variants from native mCherry sequence shown in this table, except cp184 (not determined), have the same excitation (587 nm) and emission (610 nm) maxima as mCherry. The excitation and emission maxima of the cp193g7, with 6 amino acid mutations, are 580 nm and 602 nm, respectively [19].bExtinction coefficients were measured by alkali-denatured chromophore method.cQuantum yields were measured using mCherry as the reference standard.dRelative brightness of chromophore (extinction coefficient × quantum yield) was compared with mCherry (91,000×0.22).eFluorescence of cp-mCherry relative to mCherry with fixed protein concentration (BCA assay).fOur data; the published data are 72,000 [7], 78,000 [9].gPublished values [18], [19], which were based on the protein quantification (absorption at 280 nm).iNot determined.