Bacteriophage infection and killing of intracellular Mycobacterium abscessus

Mycobacterium abscessus is a nontuberculous mycobacterium (NTM) that contributes to the decline and death of patients with lung diseases such as cystic fibrosis and other muco-obstructive airway diseases. M. abscessus is challenging to treat due to its extensive antibiotic resistance and ability to survive inside mammalian cells. An alternative to antibiotics is the therapeutic use of bacteriophages (phages). There are recent cases of phage therapy being used to treat M. abscessus infections in people under compassionate-use conditions. However, little is known about the ability of phages to kill bacteria, such as M. abscessus, that reside in an intracellular environment. Here, we used M. abscessus strains and phages from recent phage therapy cases to determine if phages can enter mammalian cells and if they can infect and kill intracellular M. abscessus. Using fluorescence microscopy, we demonstrate phage uptake by macrophages and lung epithelial cells, and we further demonstrate phage..., , , # Data from Bacteriophage Infection and Killing of Intracellular Mycobacterium abscessus

**Figure 2ABCD**
Fig2ABCD.csv: CSV file reporting raw data for bacterial colony forming unit (CFU) counts from time points between day 0 and day 5 post-infection for Figure 2ABCD. Raw values were transformed log10 for publication.
**Figure 4BCD**
Fig4BCD.csv: CSV file reporting raw data for quantification of phage puncta per mammalian cell in individual fields of view for Figure 4BCD. Values represent the percent of mammalian cells with intracellular phage.
**Figure 5BCD**
Fig5BDC.csv: CSV file reporting raw data for quantification of bacteria expressing mCherry as an indicator of phage infection for Figure 5BCD. Values represent percent reporter phage infection of intracellular *M. abscessus*.
**Figure 7ABC**
Fig7ABC.csv: CSV file reporting raw data for bacterial colony forming unit (CFU) counts from time points at day 0 and day 2 post-infection for Figure 7ABC. Raw values were transformed log10...