Single-cell transcriptome analyses reveal endothelial cell heterogeneity in tumors and changes following anti-angiogenic treatment

To understand tumor stromal cell heterogeneity focusing on tumor endothelial cells and tumor-associated fibroblasts, we isolated single cells from COLO205 tumor xenograft grown sub-cutaneously in SCID mice. To enrich stromal cell content, we removed vast majority of tumor cells by a depletion protocol using anti-CD24 and anti-E-Cadherin antibodies. The remaining single cells were profiled by single cell RNAseq Overall design: Tumors were dissociated, sorted into single cells, and profiled by 1 chromium. Single cell 3'' library, Gelbeads and multiplex kit were made by using 10x Genomics platform.