Transcriptome analysis after deletion of single miR-1/133a clusters.

Affymetrix GeneChip transcriptome analysis after loss of single miR-1/133a clusters revealed only limited regulation of miR-1/133a target molecules. Of note Kcne1 was upregulated in both knock-out models, however many other previously described miR-1 or miR-133a targets were not regulated after loss of roughly 50% miR-1 or miR-133a. Consistent with upregulation of SRF in miR-1-1/133a-2 mutant animals we observe upregulation of smooth muscle/fetal gene program markers in these mutants. Kcnd2 has been described to be a direct transcriptional target of Irx5. The comment indicates that the gene belongs to the smooth muscle gene program (sm) or is a predicted target of miR-1/133a regulation (t).Transcriptome analysis after deletion of single miR-1/133a clusters.