Global quantitative MS proteome analysis of in vitro activated murine primary B cells of Dis3G766R/+ and Dis3+/+ genotypes

DIS3 is a ribonucleolytic subunit of the exosome complex responsible for RNA degradation in the nucleus, predominantly affecting steady state levels of non-coding transcripts. The effect of the mild mutant Dis3 G766R allele, clinically related t multiple myeloma, in a heterozygous state on protein-coding transcripts is negligible. In this experiment, we assayed the proteome of in vitro activated primary B cells 72 hours after their activation with LPS (20 µg/ml) and IL-4 (20 ng/ml). TMT-based global quantification of protein levels was performed in Dis3G766R/+ and Dis3+/+ samples in triplicates per condition. This dataset is directly related to a transcriptomic analysis available under a GEO accession number GSE155631 containing RNA-seq and DRIP-seq data of corresponding samples