Single-cell ATAC-Sequencing of adipose Treg cells

Systematic characterizations of adipose Treg subsets and their in vivo roles remain uncommon. The goal of this study is to reveal the heterogeneity and chromatin landscape of adipose Treg cells. Using single-cell ATAC-seq to map adipose Treg cells, we identified different adipose subsets. Strikingly, our scATAC-seq analysis revealed a strong enrichment of key transcription factor motifs, which may play function in fate specification. By gain and loss of function studies, we further demonstrated adipose Treg subsets display disparate physiologic influences. Our findings have important implications for understanding phenotypic diversity of tissue Tregs in vivo. Overall design: Around 60,000 TCRß+CD4+CD25+YFP+ adipose Treg cells from 4-month old male Foxp3Cre mice were subjected to nuclei isolation according to the demonstrated protocol: Nuclei Isolation for Single Cell ATAC Sequencing (10x Genomics). scATAC-seq libraries were prepared according to the Chromium Single Cell ATAC Reagent Kits User Guide (10x Genomics; CG000168 Rev A).