Silencing of KDM6A regulates TGF-β signaling pathway in MCF10A cells

Massive parallel sequencing projects identified KDM6A as a tumor suppressor gene with recurrent loss-of-function mutations multiple cancers. However, in breast cancer, reports on the functional role of KDM6A is paradoxical or even puzzling, suggesting the role of KDM6A may be context dependent. We sought to investigate the functional role of KDM6A in triple negative breast cancer with using MCF10A cells. KDM6A (also known as UTX) was silenced in MCF10A cells using single guide RNA (sgRNA) mediated genomic editing using CRISPR/Cas9 system. Whole RNA was extracted using RNeasy mini extraction kit (74106, Qiagen). RNA-seq was performed with Illumina Hiseq4000 platform.