Impact of LXR activation on alternatively activated human macrophages

To investigate how LXR activation potentiates expression of Th2 cytokine-dependent genes in primary human macrophages, we pulsed macrophages with synthetic LXR ligand T0901317 then polarized cells to alternatively activated macrophages with IL-4 or IL-13. Overall design: Human monocytes derived from three individual donors were differentiated into naïve macrophages with RPMI 1640 medium containing 5% AB-positive human serum for 7 days. Naive macrophages were pulsed with T0901317 (1 µM) for 3 hours, then media was aspirated and cells were polarized to alternatively activated macrophages with IL-4 (5 ng/ml) or IL-13 (10 ng/ml) for 24 hours.