Vibrio cholerae vpsT microarray analysis. Vibrio cholerae

Rugose V. cholerae cells with an in-frame deletion of vpsT (VCA0952) were used in this study. Cells containing pBAD-Myc/His-B vector, or the vector containing a wild-type copy of vpsT, or vpsT with point mutations M17D, D60A, R134A, or I141E were grown to exponential phase and expression profiles compared. Overall design: Three biological replicates were performed for each strain and dye swaps were performed for each treatment. Reference sample consisted of V. cholerae cells with an in-frame deletion of vpsT carrying the pBAD-Myc/His-B vector.