The role of TERF2IP (Telomeric repeat binding factor 2-interacting protein) in regulating disturbed flow-induced changes of gene expression

To understand the function of TERF2IP in ECs, we isolated total RNAs from human aortic endothelial cells (HAECs) transfected with control or TERF2Ip siRNA and transcriptionally profiled them. HAECs were transfected by control siRNA or TERF2IP siRNA, and after 48 hrs of transfection HUVECs were exposed to disturbed flow (d-flow). After 24 hrs of disturbed flow RNA was extracted for futher microarray processing. We have the following four groups (1. Control siRNA under static condition (siCont), 2. Control siRNA under d-flow (siCont + DF), 3. TERF2IP siRNA under d-flow (siTERF2IP), and 4. TERF2IP siRNA under d-flow (siTERF2IP + DF), and each group have three samples and total twelve samples were analyzed.