3D-SpermFlagella: 3D+t human sperm flagellum centerline dataset

Summary The 3D-SpermFlagella dataset contains the three-dimensional (3D) coordinates of sperm flagellum centerlines over time (t), derived from the spatiotemporal (3D+t) image dataset 3D-SpermVid. It comprises 135 human sperm cells, each tracked for 1 -3.5 seconds. The number of time points varies depending on the specific acquisition. Cells were incubated under two experimental conditions: 49 under non-capacitating conditions (NCC) and 86 under capacitating conditions (CC). For each time point (i.e., each volumetric image), the flagellum centerline is represented as a sequence of 3D coordinates, resulting in a spatiotemporal reconstruction of flagellar motion. The centerline coordinates for each cell are provided in both micrometers and voxels. Supplementary material Supplementary videos showing the 3D centerlines can be found in: https://doi.org/10.5281/zenodo.18434653