48hrs and 72hrs dose-percent response data.xlsx

<i>Bemisia tabaci</i> populations were collected from greenhouses located in Aydıncık, Kazanlı, Kocahasanlı, Silifke and Sipahili districts in southern Turkey (Mersin province). Three neonicotinoid insecticides—acetamiprid 207g/kg (Mospilan™, Sumi Agro Turkey, Istanbul, Turkey), imidacloprid 350 g L^-1 (Confidor™, Bayer Crop Sciences, Leverkusen, Germany), and thiamethoxam 240 g L^-1 (Actara™, Syngenta, Izmir, Turkey)—and three non-neonicotinoid insecticides—spinetoram 120 g L^-1 (Radiant™, Dow AgroSciences, Istanbul, Turkey), spinosad 480 g L^-1 (Laser™, Dow AgroSciences, Istanbul, Turkey), and sulfoxaflor 50% W/W (Transform™, Dow AgroSciences, Istanbul, Turkey)—were used in the bioassays. Serial dilutions were prepared for each insecticide using distilled water to determine population specific concentrations. A 0.1 g L^-1 Triton X-100 was included in the insecticide concentrations. A total of five to seven concentrations that caused nearly 10% to 90% mortality were determined (IRAC, https:// irac-online.org).The method described by the Insecticide Resistance Action Committee (IRAC) was used to determine the resistance status of whitefly populations (IRAC, https:// irac-online.org). Bean leaf-discs (3cm in diameter) that were cut using a cork-borer from insecticide-free bean plants were immersed in different concentrations of insecticides for 10 sec. The leaf-discs were allowed to dry for 30 min on paper tissues and then were placed (with their abaxial surface up) on thin sterile agar layers (10 g/L) in 6mm Petri dishes. Fifteen <i>B. tabaci</i> adult individuals were transferred to each leaf-disc using a mouth aspirator and confined using a close-fitting ventilated lid. The lids were previously covered with mesh suitable for keeping whiteflies. Subsequently, the test units were transferred to a controlled condition (Nüve® Incubator, 25±2ᵒC, 70±5%RH, and 16L:8D). Test units, which were treated with only distilled water mixed with 0.1 g L-1 Triton X-100, were used as control. Mortality assays for control were conducted independently for each insecticide. Each concentration was replicated 10 times (150 adults for each concentration). Mortality was evaluated after 72 hrs following pesticide treatments using a binocular microscope. Whitefly individuals that were unable to flip themselves up or fly for a prolonged period of time were considered dead (Caballero, Cyman &amp; Schuster, 2013). This data can be used to generate LC50, LC90, LC25 etc values through probit analysis.