Gene expression profile at single cell level of whole embryonic (E16.5) and early postantal (P3) mouse liver

To study molecular differences betweeem wild-type and cholestatic livers of a Jag1Ndr mouse model of Alagille syndrome (Andersson, Chivukula, Hankeova, et al. Gastroenterology, 2018;154(4):1080-1095), we used single cell RNA sequencing (scRNA-seq) of prenatal (E16.5) and early postnatal (P3) livers. Overall design: Liver cells of Jag1+/+, Jag1Ndr/+ , and Jag1Ndr/Ndr mice (2 individuals per genotype and stage) were enzymatically dissociated, dead cells removed on MACS, and directly processed for scRNAseq with 10X Genomics, followed by library preparation and sequencing on Illumina NextSeq550, and S4 NovaSeq.