Coexpression of gut reporter GFP and intestinal granules in embryos defective in P2-EMS signaling.

Number of embryos (N) expressing one of two E lineage-specific reporters: Pend-1::gfp::h2b (end-1 reporter) or Psdz-23::gfp::h2b (sdz-23 reporter). The end-1 reporter was tested in wildtype (WT) embryos (N = 60), embryos mutant in the Wnt pathway: mom-1(or10), N = 9; mom-1(se2), N = 13; mom-3(or78), N = 25; mom-5(RNAi), N = 22; or embryos mutant in the MAPK pathway: mom-4(ne19), N = 17; mom-4(or39), N = 21; mom-4(or11), N = 10. The sdz-23 reporter was tested in WT, N = 44; mom-1(or10), N = 16; mom-3(or78), N = 41; and mom-4(ne19), N = 4.Reporter expression results (GFP+/−) are correlated with gut granule development (GG+/−) for each individual embryo, generating 4 classes: GFP+/GG+, GFP−/GG+, GFP+/GG−, and GFP−/GG−.Coexpression of gut reporter GFP and intestinal granules in embryos defective in P2-EMS signaling.