Gene expression profile of N171-HD82Q hippocampus and cerebellum

Transcriptional dysregulation is an important early feature of polyglutamine diseases. One of its proposed causes is defective neuronal histone acetylation, but important aspects of this hypothesis, such as the precise genomic topography of acetylation deficits and the relationship between transcriptional and acetylation alterations at the whole-genome level, remain unknown. We examined here the genome-wide correlation of histone acetylation and gene expression defects in a mouse model of early-onset Huntington's disease. Hippocampi and cerebella were dissected from 10- and 20-weeks old transgenic mice alongside with wild-type littermates. Total RNA was extracted and hybridized to Affymetrix MoGene 1.0 ST arrays. One array consisted on a pool of 3-4 RNA samples and three arrays were used per condition (genotype-tissue-age). This is the gene expression component of the study only.转录失调是多聚谷氨酰胺疾病（polyglutamine diseases）的重要早期病理特征。该病症的潜在致病机制之一被认为是神经元组蛋白乙酰化功能异常，但该假说仍有诸多关键科学问题尚未阐明，例如乙酰化缺陷的确切基因组拓扑分布特征，以及全基因组层面转录组改变与乙酰化修饰改变之间的内在关联。本研究针对早发性亨廷顿舞蹈症（early-onset Huntington's disease）小鼠模型，分析了全基因组范围内组蛋白乙酰化异常与基因表达缺陷之间的相关性。研究样本取自10周龄与20周龄的转基因小鼠及其同窝野生型对照小鼠的海马体与小脑。提取总RNA后，将样本与Affymetrix MoGene 1.0 ST基因芯片进行杂交实验。每张芯片对应3-4份RNA样本的混合池，每一种实验条件（基因型-组织-年龄组合）均设置3张重复芯片。本数据集仅包含该研究的基因表达分析部分。