consensus sequences drafted from microbial communities that were characterized with ONT 16S rRNA gene sequencing

Consensus sequences generated by CONCOMPRA, NGSpeciesID, and amplicon_sorter from (1) a synthetic bacterial community composed of 20 equally abundant bacteria (16S MOCK; https://www.atcc.org/products/msa-1002) or (2) the ZymoGut Microbiome Standard, a microbial community mimicking the human gut microbiome (Gut Microbiome Standard; https://files.zymoresearch.com/datasheets/ds1712_zymobiomics_gut_microbiome_standard_data_sheet.pdf). The first was sequenced twice, on both a R9 and and R10 flow cell (indicated by the folder structure). For CONCOMPRA, all the consensus sequences ('CONCOMPRA.all_consensus') are given as well as the non chimeric sequences only ('CONCOMPRA.noglobal_nolocalchim.consensus.sequences'). The full-length of the 16S rRNA gene was amplified with the primer set 27F and 1492R.Sequencing details as well as the data processing steps are described in: Stock, Willem, et al. "Breaking free from references: a consensus-based approach for community profiling with long amplicon nanopore data." <i>bioRxiv</i> (2024): 2024-07. ( https://www.biorxiv.org/content/10.1101/2024.07.04.602031v1 )<br>