RNA-Seq of single cells from the mouse lumbar dorsal root ganglion

In order to establish a consensus catalog of dorsal rott ganglion cell types, we used comprehensive transcriptome analysis of single cells for unsupervised identification and molecular classification of sensory neurons independent of any a priori knowledge of sensory subtypes. RNA-Seq was performed on 799 dissociated single cells dissected from the mouse lumbar dorsal root ganglion distributed over a total of nine 96-well plates为构建背根神经节（dorsal root ganglion）细胞类型的共识分类目录，本研究借助单细胞全转录组分析技术，在无需预设感觉神经元亚型先验知识的条件下，对感觉神经元实施无监督鉴定与分子分型。实验针对从小鼠腰段背根神经节中解离得到的799个单个细胞开展RNA测序（RNA-Seq），这些细胞被分布于总计9块96孔板中。