T cell signaling proteins 3D confocal microscope movies

This dataset contains 3D confocal microscope movies of T cell clones expressing different eGFP-tagged proteins as they undergo synapse formation with antigen presenting cells (APCs). Each movie shows a field of T cells and APCs every 20 s for 6 minutes after the cells are mixed. Each frame shows some cells in various stages of antigen presentation. The movies are for two parental T cell clones, four stimulation conditions and sixteen proteins. The acquisition and analysis of the movies is described in D. J. Clark, L.E. McMillan, S.L. Tan, G. Bellomo, C. Massou, H. Thompson, L. Mykhaylechko, D. Alibhai, X. Ruan, K.L. Singleton, M. Du, A.J. Hedges, P.L. Schwartzberg, P. Verkade, R.F. Murphy, and C. Wülfing (2019) Transient protein accumulation at the center of the T cell antigen presenting cell interface drives efficient IL-2 secretion. eLife 8:e45789. https://doi.org/10.7554/eLife.45789