Increased DNA replication length offsets developmental defects induced by hypomorphic DNA methylcytosine methyltransferase activity [RNA-Seq]

Dnmt1 is a key methyltransferase involved in the maintenance of DNA methylation. To determine whether developmental defects induced by hypomorphic DNA 5-methylcytosine methyltransferase activity can be offset by increasing DNA replication time, we produced fish that were mutant for both dnmt1 and DNA polymerase epsilon (pole1) and compared their whole genome bisultite sequencing and gene expression profiles to WT ones. We further determine that the observed rescue effect by pole1 is specific to dnmt1 by comparing whole-genome bisulfite sequencing and gene expression profiles of mutants for the Methionine Adenosyltransferase 2A (mat2aa) and microchromosome 10 (mcm10) Characterisation of gene expression profiles of dnmt1/pole1 single or double mutant fish, mat2a/pole1 single or double mutant fish or mcm10 single mutant fish via RNA-Seq