RNASeq of skin tissues by Mesenchymal stem cells treatment on DNCB induced mice model

Evaluate the therapeutic effects of different delivery route of MSC treantment in DNCB induced atopic dermatitis mice model. Overall design: 12 Balb/C mice were randomly divided into 4 groups and the hairs of dorsal skin were shaved two days before sensitization. For sensitization, 1% DNCB was applied to the shaved area of the dorsal skin. Mice of the solvent control group were treated with vehicle only (acetone: olive oil = 3:1 v/v). Subsequently, the dorsal skin were repeatedly challenged with 0.5% DNCB solution. Each mice in the MSC treatment group received the dose of 2×106 MSCs in 100µL culture solution. For scMSC group, 100µL MSC solution were injected subcutaneously in the dorsal skin area. For scivMSC group, 50µL MSC solution were injected subcutaneously in the dorsal skin area and another 50µL MSC solution were injected into tail vein.