Effect of SASP factors on mouse intestinal organoids [bulk RNA-seq]

We report bulk RNAseq data from the mouse intestinal organoids cultured in different conditions. Differential gene expression analysis revealed a large overlap in induced genes between Wnt5a and SCM, consistent with a major contribution of non-canonical Wnt signaling to the SCM-induced cystic organoid phenotype. Of genes differentially regulated by Wnt5a and SCM, about half were Ptk7 dependent, which displayed strong enrichment for TEAD binding motifs. Crypts isolated from 6 different mice (animal origin ID 1-6) were used to establish the intestinal organoid culture. Freshly passaged crypts from these organoids were cultured in complete media, QCM or SCM (with or without anti-Ptk7), and complete media with Wnt5a (with or without anti-Ptk7)