Transcription profiling of pancreata from Ptf1a-Cre;LSL-KrasG12D and Ptf1a-Cre;LSL-KrasG12D;RelAfl/fl mice

Oncogene-induced senescence in early mPanIN lesions depends on intact RelA function in vivo. We investigated the difference in gene expression between KrasG12D pancreata with and without deletion of RelA. Pancreata from 7 day old mice were prepared, RNA was isolated and Affymetrix microarray expression analysis was performed.体内早期小鼠胰腺上皮内瘤变（mouse Pancreatic Intraepithelial Neoplasia, mPanIN）病变中的癌基因诱导衰老（Oncogene-induced senescence）依赖于RelA的完整功能。本研究探究了KrasG12D突变背景下，RelA缺失与RelA完整的胰腺组织之间的基因表达差异。我们制备了7日龄小鼠的胰腺组织，提取总RNA并完成了Affymetrix基因芯片表达谱分析。