Design of Spiro[2.3]hex-1-ene, a Genetically Encodable Double-Strained Alkene for Superfast Photoclick Chemistry
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https://figshare.com/articles/dataset/Design_of_Spiro_2_3_hex_1_ene_a_Genetically_Encodable_Double_Strained_Alkene_for_Superfast_Photoclick_Chemistry/2030190
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资源简介：
Reactive yet stable alkene reporters
offer a facile route to studying
fast biological processes via the cycloaddition-based bioorthogonal
reactions. Here, we report the design and synthesis of a strained
spirocyclic alkene, spiro[2.3]hex-1-ene (Sph), for an accelerated
photoclick chemistry, and its site-specific introduction into proteins
via amber codon suppression using the wild-type pyrrolysyl-tRNA synthetase/tRNACUA pair. Because of its high ring strain and reduced steric
hindrance, Sph exhibited fast reaction kinetics (k2 up to 34 000 M–1 s–1) in the photoclick chemistry and afforded rapid (<10 s) bioorthogonal
protein labeling.
创建时间：
2015-12-17
