Transcriptome expression of human bone marrow mesenchymal stem cell (hBMSCs) during osteogenesis

In this study, we performed the high-throughput sequencing of hBMSCs between day 0 and day 21 during induction of osteogenic differentiation, followed by analysis of the different expression in whole transcriptomes. Overall design: Bone marrow tissue taken from the volunteers was isolated for hBMSCs. Flow analysis and multidirectional differentiation induction were used to identify stem cell characteristics and differentiation potential of hBMSCs. The third passage of hBMSCs were planted on 6-well plates and cultured in osteogenic differentiation induction medium. The induction medium was changed every three days. At day 0 and day 21, the cells were digested and collected for sequencing.