Next-generation sequencing to map trinucleotide repeats at single base-pair resolution. Homo sapiens

We have developed a new method using the Next-generation sequencing to map trinucleotide repeats at single base resolution. As a proof of evidence,we first apply on human embryonic stem cells. We have generated 32.3Gb single-end sequence data using the Illumina Hiseq 2000.Phred quality score are converted from Illumina to Sanger format and the last base is trimmed. No other filtering has been done prior to submission.