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Quantification data for article entitled "ATG9 vesicles comprise the seed membrane of mammalian autophagosomes"

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Zenodo2023-04-05 更新2026-05-26 收录
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Membrane fractionation, immunoprecipitation, and <em>in vitro</em> experiment densitometric quantification datasheets for quantified data presented in the research article entitled "ATG9 vesicles comprise the seed membrane of mammalian autophagosomes". Membrane fractions (Figures 3, 4, S4, and S5) were from WT cells (starvation + bafiomycin A1 and basal conditions), ATG2 DKO cells, FIP200 KO cells, ATG9A KO cells, ATG3 KO cells, and ATG4 QKO cells (all KO cells under basal conditions). Immunoprecipitations (Figures 6 and S5) were from WT cells (starvation + bafiomycin A1) and ATG2 DKO cells (basal). Guanidine hydrochloride (GdnHCl) sequential immunoprecipitations (Figure 6) were from WT cells (starvation + bafiomycin A1). <em>In vitro</em> experiments (Figure S5) were using recombinant proteins on reconstituted liposomes.

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2023-04-04
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