HOT-288 SKQ201615S Fluorometric Chlorophyll

These Simons Collaboration on Ocean Processes and Ecology (SCOPE) data products were produced by the University of Hawaiʻi at Mānoa with support from the Simons Foundation. The HOT-288 cruise on the R/V Sikuliaq was performed from November 25th to 29th, 2016 at Station ALOHA (22.75, -158) north of Oahu, Hawaii. Fluorometric chlorophyll samples were collected from rosette niskin bottles at specific depths and filtered at low vacuum through a glass fiber filter. Samples were then flash frozen in liquid nitrogen and stored in a histoprep capsule at -80°C until analysis. Post-cruise, the filters were extracted using 90% acetone for a total of 48 hours in a -20°C freezer, covered to prevent the photodegradation of pigments. Upon extraction, samples were centrifuged to settle filter particulates and gently poured into a glass cuvette. Samples were then run on a Turner 10-AU Fluorometer. Data was corrected for average glass fiber filter blank values and is reported as the average of triplicate samples. Sample with no cast/niskin was collected from underway flowthrough system. The timestamp is in UTC.