Expression data from sympathetic nervous system of KI Alk F1178L mice

Neuroblastoma is an embryonal neoplasm that remains of dramatic prognosis in its aggressive forms. Activating mutations of the ALK tyrosine kinase receptor have been identified in sporadic and familial cases of this cancer. We generated knock-in mice carrying an F1178L Alk mutation corresponding to the ALK F1174L mutation observed in neuroblastoma patients. We used microarrays to detail the global programme of gene expression underlying the impact of ALK F1178L mutation on sympathetic nervous system ganglia. We selected sympathetic nervous system ganglia (superior cervical and stellate ganglia) for RNA extraction and hybridization on Affymetrix microarrays. We profiled a total number of 12 Po (4 Wt; 4 Hz; 4 Ho) and 5 P18 (3 Wt; 2 Hz)神经母细胞瘤（Neuroblastoma）是一类胚胎源性肿瘤，其侵袭性亚型仍具有凶险的预后。目前已在该癌症的散发性与家族性病例中，鉴定出间变性淋巴瘤激酶酪氨酸激酶受体（ALK tyrosine kinase receptor）的激活突变。我们构建了携带F1178L Alk突变的基因敲入小鼠（knock-in mice），该突变对应神经母细胞瘤患者中检出的ALK F1174L突变。我们利用基因芯片（microarrays）解析了ALK F1178L突变作用于交感神经节时的全局基因表达程序。我们选取交感神经节（包括颈上神经节与星状神经节）进行RNA提取，并在Affymetrix基因芯片上完成杂交实验。本研究共对12只Po（4只野生型[Wild Type, Wt]、4只杂合子[Heterozygous, Hz]、4只纯合子[Homozygous, Ho]）以及5只P18（3只Wt、2只Hz）小鼠开展了基因表达谱分析。