Microarray gene expression data from sorted lung epithelial cells during heterotypic immunity

Previous pneumococcal experience establishes lung-resident IL-17A-producing CD4+ memory TRM cells that accelerate neutrophil recruitment against heterotypic pneumococci. Herein, we unravel a novel crosstalk between CD4+ TRM cells and lung epithelial cells underlying this protective immunity. Genome-wide expression analyses of lung epithelial cells in CD4+ cell sufficient and CD4+ cell deficient heterotypic immune murine lungs revealed a significant remodeling of the epithelial transcriptome of infected lungs due to infection history, ~80% of which was CD4+ cell-dependent. To achieve a more comprehensive view of epithelial remodeling, and to identify the extent to which this event is CD4+ T cell-dependent, we performed transcriptomic analyses on sorted CD45-EpCAM+ cells from lungs of heterotypic immune or naïve mice treated with GK1.5 (Het GK1.5) or control IgG (Saline IgG or Het IgG). All mice had 7 hours of Sp3 pneumonia in these analyses. We aimed to determine whether prior experience with unrelated serotypes altered the epithelial transcriptome during pneumonia, and if this depended on CD4+ T cells.