Epigenetic modification of GR enhancers by SETD1A/COMPASS complex in murine macrophages

Nuclear interaction studies by ChIP coupled with mass spectrometry identified the COMPASS/SETD1A complex as interaction partner of the glucocorticoid receptor (GR) in murine bone marrow-derived macrophages (BMDMs). Here, we profiled the occupancy of SETD1A and CXXC1, two subunits of the COMPASS/SETD1A complex in murine macrophages after LPS and Dex+LPS stimulation. We show, that GR recuits SETD1A and CXXC1 to GR enhancer and that SETD1A recruitment does correlate with changes in H3K4methylation at some of those enhancers. Overall design: We profiled changes in SETD1A and CXXC1 occupany as well as changes in H3K4methylation and H3K27Ac in murine bone marrow-derived macrophages after challenge with LPS or Dex+LPS. All ChIP-Seq experiments were done in duplicates or triplicates, partially including Drosophila melanogaster chromatin as spike-in control.