Discordant population structure among rhizobium divided genomes and their legume hosts

Symbiosis often occurs between partners with distinct life history characteristics and dispersal mechanisms. Many bacterial symbionts have genomes comprised of multiple replicons with distinct rates of evolution and horizontal transmission. Such differences might drive differences in population structure between hosts and symbionts and among the elements of the divided genomes of bacterial symbionts. These differences might, in turn, shape the evolution of symbiotic interactions and bacterial evolution. Here we use whole-genome resequencing of a hierarchically-structured sample of 191 strains of Sinorhizobium meliloti collected from 21 locations in southern Europe to characterize the population structures of this bacterial symbiont and its host plant Medicago truncatula. Sinorhizobium meliloti genomes showed high local (within-site) variation and little isolation by distance. This was particularly true for the two symbiosis elements pSymA and pSymB, which have population structures that..., Here we use whole-genome resequencing of a hierarchically-structured sample of 191 strains of Sinorhizobium meliloti collected from 21 locations in southern Europe to characterize the population structures of this bacterial symbiont and its host plant Medicago truncatula.
We extracted DNA from cultures of S. meliloti grown in liquid TY media using Qiagen DNeasy (Hilden, Germany) and sent samples to the DOE Joint Genome Institute (JGI) for sequencing (Berkeley, CA, USA). JGI prepared a paired end library for each strain, and sequenced samples on an Illumina HiSeq-2500 1TB platform (101nt read length; Illumina, Inc., San Diego, CA, USA). Of the 199 strains submitted to JGI, we received high-quality whole genome sequences for 166. We re-grew the remaining 33 strains from frozen cultures (as above), and extracted DNA using the Zymo Quick-DNA kit for Fungi or Bacteria (Irvine, CA, USA). These samples were sequenced (2 X 150 or paired end 150 nt read length) on the Novaseq 6000 platform (Illu..., ,