Identification of genes showing altered DNA methylation and gene expression in the renal proximal tubular cells of rats treated with ochratoxin A for 13 weeks

The present study investigated comprehensive gene profiles regarding DNA methylation and gene expression in rat renal tubules after 13-week administration of ochratoxin A (OTA), a karyomegaly-inducing renal carcinogen. OTA-specific genes were obtained by excluding those responding to 3-MCPD, a renal carcinogen not inducing karyomegaly.本研究针对可诱导核肿大的肾脏致癌物赭曲霉毒素A（ochratoxin A, OTA），分析了经13周OTA染毒后大鼠肾小管（renal tubules）中涵盖DNA甲基化（DNA methylation）与基因表达（gene expression）的全基因谱。通过剔除对3-氯-1,2-丙二醇（3-MCPD）产生应答的基因，最终得到OTA特异性基因；其中3-MCPD为不具备核肿大诱导能力的肾脏致癌物。