NGS results of genome editing in HIV-1-Infected Jurkat cells using crRNA array-loaded EMT-Cas12a

This project developed an exosome-mediated targeted CRISPR-Cas12a delivery system (EMT-Cas12a), and we designed seven anti-HIV-1 crRNAs(L4, G6, G16, P7, P88, T1, E1) targeting conserved viral genomic regions (LTR, Gag, Pol, Tat, Env). We conducted HIV-1 genotyping via NGS to map mutational profiles in the HIV-1 genome. Jurkat cells infected with HIV-1 NL4-3 were treated once with diferent EMT-Cas12a (10^6 particles per cell), followed by 5-day culture. Cellular DNA was extracted, and targeted HIV-1 regions (L4, G6, G16, P7, P88, T1, E1) were PCR-amplified and subjected to NGS.