Eco-friendly fluorimetric approaches for the simultaneous estimation of the co-administered ternary mixture: etoposide, moxifloxacin, and nalbuphine

Antineoplastic drugs as etoposide (ETO) are widely utilized in leukemia
cancer. Patient with leukemia cancer has a relative infection with
pneumonia treated by fluoroquinolones as moxifloxacin HCL (MOX). Because
opioid analgesic as nalbuphine HCL (NAL) does not have a ceiling dose, it
is used to manage the distasteful sensory in leukemia cancer. Green
chemistry advances innovative design to keep a healthy environment.
Consequently, innovatory green processes for synchronous
spectrofluorimetric quantification of a ternary mixture of antineoplastic,
fluoroquinolone, and opioid analgesic drugs. The present study provides
two eco-friendly synchronous fluorimetric processes (conventional and
first derivative), conventional for MOX and the first derivative for ETO
and NAL quantification. Conventional synchronous fluorimetric of MOX was
monitored at 371 nm, while the first derivative synchronous fluorimetric
of ETO and NAL was measured at 257 and 273 nm, respectively, in ethanol.
The synchronous fluorescence was scanned at  λ of 60 nm for both
processes. Environmentally friendly solvent as ethanol has a major role in
the sensitivity, reproducibility, and quantitation of innovative
processes. The outcomes of these designed environmentally friendly
processes, a linear correlation was described in the ranges of 0.04-0.40,
0.1-1.0, and 0.5-5.0 g/mL for MOX, ETO, and NAL, respectively.
Accordingly, several pharmaceutical dosages were achieved within the range
simply and precisely. Additionally, synchronous assessment of ETO, MOX,
and NAL in the biological fluid was attained by facile protein
precipitation technique. These innovatory green processes were evaluated
as accurate and precise processes regarding ICH roles. Greener innovative
synchronous fluorimetric processes were corroborated by applying the
National Environmental Methods Index, Analytical Eco-scale, and Green
Analytical Procedure Index, which provides a novelty of the current study.