Microarray analysis of mouse gene expression in metronomic cyclophosphamide-treated rat glioma 9L xenografts implanted in scid mice

Cyclophosphamide (CPA) treatment on a six-day repeating metronomic schedule induces a dramatic, innate immune cell-dependent regression of implanted gliomas. However, little is known about the underlying mechanisms of innate immune cell mobilization and recruitment, or about the role of DNA damage and cell stress response pathways in eliciting the anti-tumor immune responses linked to tumor regression. To address these questions, we compared untreated and 6-day metronomic cyclophosphamide-treated rat 9L gliosarcoma xenografts by mouse microarray analysis to identify responsive mouse (host) cell-specific factors.采用以6天为周期的节律性给药方案给予环磷酰胺（Cyclophosphamide, CPA）治疗，可诱导植入型胶质瘤发生显著的、依赖先天免疫细胞的消退。然而，目前对于先天免疫细胞的动员与募集的潜在机制，以及DNA损伤与细胞应激反应通路在介导与肿瘤消退相关的抗肿瘤免疫应答中所发挥的作用，仍知之甚少。为解答上述问题，本研究通过小鼠基因芯片分析（microarray analysis），对比了未经处理的大鼠9L胶质肉瘤异种移植瘤与经6天节律性环磷酰胺处理的同类移植瘤，以鉴定响应的小鼠（宿主）细胞特异性因子。