High-throughput measurements of fitness effects of mutations in the yeast U3 snoRNA

We used a molecular barcoding approach to quantify the fitness of ~60,000 randomly mutated variants of the Saccharomyces cerevisiae U3 snoRNA (the data were further used to calculate a map of intragenic epistatic interactions within this RNA molecule, as descibed in Puchta et al. 2016)本研究采用分子条形码（molecular barcoding）技术，对酿酒酵母（Saccharomyces cerevisiae）U3小核仁RNA（U3 snoRNA）的约6万个随机突变变体的适合度进行定量测定。该数据后续被用于绘制该RNA分子内的基因内上位相互作用图谱，相关细节详见Puchta等人2016年发表的研究成果。